2018-02-12 19:13:432025-05-25 12:26:52
locus
BSU04260
BSU_04260
geneLength
2181
2184
outlinks
bsu
BSU04260
BSU_04260
Gene
Coordinates
476,558 → 478,741
476,558 478,741
The protein
Catalyzed reaction/ biological activity
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to Swiss-Prot)
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to UniProt)
The protein
Protein family
topo IA family (according to Swiss-Prot)
Type IA topoisomerase family (together with [[protein|TopB]])(according to UniProt)
The protein
Paralogous protein(s)
[[protein|TopA]]
[[this]]
Biological materials
Mutant
MGNA-C111 (topB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2109 NBRP B. subtilis, Japan]
BKE04260 (''topB''::''ermC'') (available at the [http://www.bgsc.org/ BGSC] and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
GP1864 (''topB''::''ermC'') (available in [SW|Jörg Stülke]'s lab)
GP1896 (''topB''::''cat'') (available in [SW|Jörg Stülke]'s lab)
JH642 and its derivatives: natural deletion of the 18 kb [[gene|ydzA]]-[[gene|mntH]] region encompassing the genes [[gene|ydzA]]-[[gene|topB]]-[[gene|ydaJ]]-[[gene|ydaK]]-[[gene|ydaL]]-[[gene|ydaM]]-[[gene|ydaN]]-[[gene|kimA]]-[[gene|mutT]]-[[gene|ydaP]]-[[gene|ydzK]]-[[gene|mntH ]][pubmed|18670626]
BKE04260 (Δ[[gene|topB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTTGATCACTTCTCTT, downstream forward: _UP4_TAATAAAAAAACCTGTGCCT
BKK04260 (Δ[[gene|topB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTTGATCACTTCTCTT, downstream forward: _UP4_TAATAAAAAAACCTGTGCCT
MGNA-C111 (topB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2109 NBRP B. subtilis, Japan]
GP1864 (Δ''topB''::''ermC'') (available in [SW|Jörg Stülke]'s lab)
GP1896 (Δ''topB''::''cat'') (available in [SW|Jörg Stülke]'s lab)
JH642 and its derivatives: natural deletion of the 18 kb [[gene|ydzA]]-[[gene|mntH]] region encompassing the genes [[gene|ydzA]]-[[gene|topB]]-[[gene|ydaJ]]-[[gene|ydaK]]-[[gene|ydaL]]-[[gene|ydaM]]-[[gene|ydaN]]-[[gene|kimA]]-[[gene|mutT]]-[[gene|ydaP]]-[[gene|ydzK]]-[[gene|mntH ]][pubmed|18670626]
BKE04260 (Δ[[gene|topB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTTGATCACTTCTCTT, downstream forward: _UP4_TAATAAAAAAACCTGTGCCT
BKK04260 (Δ[[gene|topB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTTGATCACTTCTCTT, downstream forward: _UP4_TAATAAAAAAACCTGTGCCT
References
Reviews
References
Original publications